Detection of DNA in polyacrylamide gels by autoradiography.

METHOD 1. Immerse the gel, together with its attached glass plate, in 7% acetic acid for 5 minutes. Remove the gel from the fixative by carefully lifting the glass plate from the fluid. 2. Rinse the gel briefly in deionized H2O. Remove excess fluid from the surface of the gel with a pad of Kimwipes. IMPORTANT Do not use absorbent paper; it will stick to the gel. 3. (Optional) Dry the gel onto a piece of Whatman 3MM paper using a commercial gel dryer. Drying the gel is generally necessary only when the gel contains DNA labeled with weak ß-emitting isotopes such as 35S or such small amounts of 32P-labeled DNA that long exposures (longer than 24 hours) are necessary to obtain an adequate autoradiographic image. 4. Wrap the gel, together with its supporting glass plate, in Saran Wrap. Smooth out any air bubbles or folds in the Saran Wrap with the broad end of a slot comb or a crumpled Kimwipe. If the DNA samples separated through the gel have been labeled with 35S, it is better not to use Saran Wrap because the plastic film will block weak ß particles. Make sure that the gel is very dry (in Step 3) and proceed to Step 5. 5. To align the gel and the film, attach adhesive dot labels marked with radioactive ink or with Detection of DNA in Polyacrylamide Gels by Autoradiography -Samb... file:///C:/Users/MILI%20JOON/Documents/protocol/Electrophoresis,...